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PURPOSE: MicroRNAs (miRNAs) are a group of small non-coding RNAs involved in different cancers, including lung cancer. Here, we aim to investigate the expression profiles of circulating miRNAs and their roles contributed to the progress of lung cancer. MATERIALS AND METHODS: The levels of circulating miRNA in lung cancer patients were investigated by miRNAs assay. Then we predicted the target genes of aberrantly expressing miRNAs by searching genetic databases. Based on the A549 cells transfected with miR-1246 mimics or miR-1246 inhibitor,we further measured the roles of miR-1246 involving in the epithelial-mesenchymal transition (EMT), migration and invasion capacities of lung cancer cells in vitro. Finally, we detected the effects of miR-1246 on glycogen synthase kinase-3β (GSK-3β)/β-catenin pathway by immunofluorescence and Western blot, respectively. RESULTS: We identified that 14 miRNAs were aberrantly expressed in the serum of lung cancer patients. Among them, miR-1246 was the most up-regulated. The cell assays indicated that miR-1246 significantly increased the migration and invasion capabilities of A549 lung cancer cells. Meanwhile, immunofluorescence analysis revealed that miR-1246 promoted EMT process of A549 cells accompanying with decreasing E-cadherin expression, while increasing vimentin and transforming growth factor β (TGF-β) expression. Furthermore, an online tool predicated that miR-1246 might bind to 3′-untranslated region of GSK-3β, which was confirmed by overexpression and knockdown of miR-1246 assays. CONCLUSION: Taken together, the study illustrates that miR-1246 regulates Wnt/β-catenin pathway through targeting GSK-3β/β-catenin, which partly contributing to tumor metastasis. MiR-1246 may play an essential role in the diagnosis and therapeutic of lung cancer.
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Humans , Blotting, Western , Cadherins , Databases, Genetic , Diagnosis , Epithelial-Mesenchymal Transition , Fluorescent Antibody Technique , Glycogen Synthase , In Vitro Techniques , Lung Neoplasms , MicroRNAs , Neoplasm Metastasis , RNA, Small Untranslated , Transforming Growth Factors , VimentinABSTRACT
This article elaborated on the necessity and importance of reform of medical education administration system in comprehensive university in promoting comprehensive development of medical education,from the point of merging of universities.It also emphasized the long-term nature and complexity of the reform process.In the meantime,it introduced the reform measures and effects of the reform in the author's university.
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Objective To investigate the possible correlation between obstructive sleep apnea hypopnea syndrome(OSAHS)and serum epithelial neutrophil-activing peptide-78(ENA-78)and VE-cadherin in pregnant patients.Methods One hundred and two pregnant women combined with OSAHS,42 of which were with hypertension,and 36 healthy pregnant women were underwent polysomnography(PSG),and their apnea hypopnea index(AHI)was recorded.Based on the apnea hypopnea index(AHI),they were divided into mild OSAHS group(36 patients),moderate OSAHS group(34 patients)and severe OSAHS group(32 patients)Serum ENA-78 and VE-cadhefin levels were detected by enzyme-linked immunosorbent assay(ELISA),and mean arterial pressure(MAP)was measured simultaneously.Results The values of AHI,MAP,ENA-78 and VE-cadherin in OSAHS with hypertension were significantly higher than those of OSAHS only group(t =13.46,11.65,12.47,13.36,all P ＜ 0.01)and the control group(t =16.72,15.31,16.35,16.73,all P ＜ 0.01).The levels of serum ENA-78 and VE-cadherin were gradually significantly decreased in the order of severe OSAHS group,moderate OSAHS group and mild OSAHS group(P ＜0.01).The AHI of OSAHS group was significantly correlated with MAP,VE-cadherin,ENA-78(r =0.75,0.71,0.68,all P ＜ 0.01).ENA-78 of OSAHS group were positively correlated with MAP and VE-cadherin(r =0.64,0.67,all P ＜ 0.01).Conclusions The condition OSAHS in pregnant patients is closely related to the levels of ENA-78 and VE-cadherin.Pregnancy combined with OSAHS may take part in the pathological process of hypertensive disorder complicating pregnancy.
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ObjectiveTo study the role and clinical significance of serum visfatin and retinol binding protein 4 (RBP4) in idiopathic sudden sensorineural hearing loss by measuring the change of their levels in the patients with idiopathic sudden sensorineural hearing loss.MethodsThe levels of visfatin and RBP4 were determined by ELISA method in the 102 idiopathic sudden sensorineural hearing loss patients who were observed at two different time points ( before and after treatment),and thirty-five patients with other neurologic diseases (20 with sciatica,16 with trigeminal neuralgia) and thirty healthy people were used as control.ResultsThe levels of visfatin and RBP4 in the serum of patients with idiopathic sudden sensorineural hearing loss after treatment [Visfatin (24.26 ± 2.17 ) μg/L; RBP4 (46.65± 5.26 ) mg/L]were markedly higher than the group with other neurologic diseases [Visfatin ( 20.67 ± 2.14 ) μ g/L; RBP4(34.37 ±5.73)mg/L] and the healthy control group[Visfatin(17.61 ±2.45) μg/L; RBP4 (24.82 ±5.24)mg/L] ( t =10.38,10.41,12.16,15.06,P ＜0.01),and it was significantly less than that before treatment [Visfatin(32.24 ± 2.37) μ /L; RBP4 ( 57.43 ± 6.19 ) mg/L] ( t =17.25,15.12,P ＜ 0.01 ).The levels visfatin and RBP4 in serum of severe group with idiopathic sudden sensorineural hearing loss [Visfatin ( 36.52 ± 2.46 ) μg/L; RBP4 (67.17 ± 5.92 ) mg/L] were markedly higher than those in the moderate group[Visfatin(28.92 ±2.26)μg/L; RBP4 (55.34±5.95)mg/L]( t =11.21,11.17,P ＜0.01).The levels visfatin and RBP4 in serum of moderate group were markedly higher than those in the mild group [Visfatin ( 25.31 ± 2.32 ) μg/L; RBP4 ( 47.48 ± 5.82 ) mg/L],all these differences were statistically significant( t =10.43,10.49,P ＜0.01 ).There was a positive correlation between visfatin and RBP4 in patients with idiopathic sudden sensorineural hearing loss ( r =0.68,P ＜ 0.01 ).ConclusionsThe levels of serum visfatin and RBP4 have instructive significance in idiopathic sudden sensorineural hearing loss treatingand prognosis estimating
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Objective To investigate the changes of high mobility group box-1 ( HMGB1 ) and VE-cadherin in serum of children with viral myocarditis and their clinical significance. Methods The serum levels of HMGB1 and VE-cadherin were detected by ELISA in 52 children with viral myocarditis, and 36 normal healthy children were enrolled as control. CK-MB was also measured in all subjects enrolled into the study. Results The serum levels of HMGB1, VE-cadherin and CK-MB in children with acute stage viral myocarditis (HMGB1 :[5.14 ±0. 23] mg/L;VE-cadherin: [5.36 ±0. 92] mg/L;CK-MB: [31.42 ± 3.22] U/L)were significantly higher than those with recovery stage viral myocarditis ( HMGB1: [ 0. 92 ± 0. 14 ] mg/L, VE-cadherin: [2. 93 ±0. 64] mg/L; CK-MB: [ 13.75 ± 3.18 ] U/L) ( t = 11.37,10. 26 and 12. 17 respectively ,Ps ＜ 0. 01 )and control (HMGB1 :[ 0. 86 ± 0. 12 ] mg/L; VE-cadherin: [ 2. 86 ± 0. 65 ] mg/L; CK-MB: [ 12. 83 ±3.04] U/L) (t = 12.06,11.19 and 12. 64 respectively,Ps ＜0.01 ). However,we found no significant difference in the serum levels of HMGB1, VE-cadherin and CK-MB between recovery stage viral myocarditis group and the control ( t = 1.26,1.19,1.43, Ps ＞ 0. 05 ). There were positive correlations between HMGB1 and VE-cadherin,CK-MB (r = 0. 73,0. 79, Ps ＜ 0. 05 ) ;and positive correlation between VE-cadherin and CK-MB (r= 0. 82, P ＜0.05). Conclusion HMGB1 and VE-cadherin may play roles in the viral myocarditis pathogenesis, which can be new prognosis factors for viral myocarditis.
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Objective To investigate the percentage of CD4 + C125 +Tregs in peripheral blood of patients with sepsis and its effect on cell immunity so as to unravel the effect of immunomodulatory therapy on it. Method Fourty patients with sepsis in ICU were randomly (random number) divided into experimental group and control group . The patients of experimental group were treated with Ulinastatin and immunoregulation agent (Thymosin αl) as well. The blood specimens were collected just before treatment, 3 days and 8 days after treatment. The percentages of CD4 + CD25 + Tregs and lymphocyte subsets were detected by using FCM (flow cytometry), and TNF-α, IL-6 and IL-10 assayed by using ELISA, and APACHE Ⅱ scores were calculated. Results Before treatment, the percentage of CD4 + CD25 + Tregs increased, and the number of lymphocytes and the percentage of T lymphocytes decreased, especially the CD4 + T lymphocytes and CD4+/CD8+ decreased more markedly, and the levels of IL-6 and TNF-α increased. After treatment,the percentage of CD4+ CD25 + Tregs was decreased, the number of lymphocytes and CD4 +/CD8 + increased, and the levels of APACHE Ⅱ score, IL-6 and TNF-α decreased especially in the experimental group decreased more significantly (P ＜ 0. 05). Conclusions The percentage of CD4 + CD25+ Tregs in peripheral blood can reflect the immune status of patients with sepsis and become a novel indicator to estimate the progress of sepsis, and the immunity and prognosis of patients. Treating the patients with Thymosin αl and Ulinastatin can raise their immunity, decrease the levels of IL-6, TNF-α and APACHE Ⅱ score and improve their prognosis.
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Objective To explore the levels and clinical significance of visfatin and VE-cadherin in the serum of children with Kawasaki diseases (KD).Methods Serum levels of visfatin and VE-cadherin were measured in 90 children with KD and 30 healthy children (control group) by ELISA,the outcome was estimated in combination with clinical symptoms.Results The serum levels of visfatin [ (32.35 ± 4.82)μg/L ] and VE-cadherin[ (5.03 ± 1.06)mg/L] in children with KD were obviously higher than those in control group[ (16.83 ±4.36) μg/L,(2.86 ± 1.02) mg/L] (t = 15.23,12.47,P ＜0.01).The serum levels of visfatin[ (36.61 ±5.22) μg/L] and VE-cadherin [ (6.14 ± 1.23) mg/L] in children with KD and CAL were obviously higher than children of KD without CAL[ (28.16 ± 4.67)g/L,(4.02 ± 1.01)mg/L] (t =8.38,7.16,P ＜0.01).In children with KD,the serum levels of visfatin and VE-cadherin were significantly correlated (r = 0.65,P ＜ 0.05).Conclusions Serum levels of visfatin and VE-cadherin increased in the acute phase of KD,and it was significantly increased in those KD children with CAL.Visfatin and VE-cadherin may be used as an important serological indicator of KD with CAL.
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Objective To observe the dynamic changes of high-sensitive C-reactive protein (hs-CRP) and visfatin in patients with acute traumatic injury of brain. Method A total of 120 patients with equal number in each gender ( n = 60) and with average age of (43.2 ± 6.2) years were admitted and treated by the neurosurgical department of ICU from August 2009 to June 2010. All patients were eligible to the diagnostic criteria of craniocerebreal injury. The clinical conditions of patients were assessed with Glasgow coma scale (C CS) at admission,and as per the scores of GCS, the patients were classified into mild degree (13- 15, n = 40), moderate degree (9 - 12, n = 40) and severe degree (3 - 8, n = 40). Another 60 subjects from those asking for health care by physical examination as control with equal number in each gender and their average age was (42.2±6.7) years.Blood samples were collected from fasted patients within 12 hours, 1d, 3d, 7d and 15 days after admission, and the levels of hs-CRP and visfatin in peripheral blood were detected. Results The levels of hs-CRP and visfatin were significantly higher in brain injury group than those in control group on the admission day (both P ＜ 0.01 ),and they both had positive relationships with severity of injury. The level of hs-CRP increased to peak on the first day of admission and visfatin increased to the peak on the 3rd day after admission. There was a correlation between levels of hs-CRP and visfatin ( r = 0.63, P ＜ 0.01 ). Conclusions hs-CRP and visfatin levels are related to the severity of acute traumatic injury of brain.
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Serum concentrations of resistin and neuron-specific enolage(NSE)were determined in 72 patients with cerebral infarction(CI),60 patients with multi-infarct dementia(MID),and 60 healthy controls by means of ELISA.Our data showed that serum resistin and NSE in the CI acute phase group were significantly higher than those in the normal control group(P＜0.01).No significant difference was found in serum resistin and NSE between the CI recovery phase group and normal controls group.Moreover,no significant difference was found in serum resistin between the MID group and CI recovery phase and normal control groups(P＞0.05);but NSE Was relatively lower(P＜0.05).This study suggest that serum resistin and NSE might be of great significance to patient's condition and prognosis.
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Soft environment of opening laboratory in key subject will deeply affect the construction and development of disciplines.Developing and innovative academic team;scientific and rigorous running mechanism,healthy and concordant academic atmosphere,active and positive behavior mode,all of the above are the main factors of soft environment of laboratory.From now on,we need try our best to improve human rights,design more efficient aims,constitute impellingly guarantee system and fine mechanism of consistent of regulations and environment,apply modern managing instrument scientificly and improve managing efficiency.
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Aim To study the effects of polyamine analogue CPENSpm on the human lung cancer line A549 in cell proliferation and apoptosis.Methods MTS was used to assay the cell proliferation,chemical analysis methods were used to determine the activities of enzymes in the polyamine metabolism,HPLC was performed to assay the intracellular concentration of polyamines,Sub-G1 and DNA fragmentation assays were used to determine the cell apoptosis.Results Treating A549 lung cancer cells by CPENSpm resulted in:①cell-growth inhibition and cell apoptosis;②inhibition of ODC(key enzyme in polyamine synthetic pathway)and activation of SSAT and SMO(key enzymes in polyamine catabolism);③great decrease of intracellular polyamine concentrations.MDL72527,the SMO inhibitor,can antagonize the effect of CPENSpm on inhibiting the proliferation of A549 cells.Conclusion CPENSpm inhibits proliferation and induces apoptosis of human A549 lung cancer cell line by interfering the polyamine metabolism,depleting intracellular polyamine contents that are need by quick-growth of cancer cells and inducing production of H2O2.